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phosphorylated mtor  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phosphorylated mtor
    Effect of corn processing on relative mRNA level for <t>mTOR</t> , 4E-BP1 , p70S6K and protein abundances of mTOR, <t>phosphorylated</t> mTOR <t>(p-mTOR),</t> eukaryotic initiation factor 4E-binding protein 1 (4E-BP1), phosphorylated 4E-BP1 (p-4E-BP1), p70 ribosomal protein S6 kinase (p70S6K) and phosphorylated p70S6K (p-p70S6K) in the longissimus thoracis of piglets. Results are presented as means ± standard error of the mean (SEM), n = 4. Data columns with different letters were significantly different ( P ≤ 0.05).
    Phosphorylated Mtor, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+mtor/pmc13053694-232-33-39
    Average 86 stars, based on 1 article reviews
    phosphorylated mtor - by Bioz Stars, 2026-09
    86/100 stars

    Images

    1) Product Images from "Modulating starch digestion kinetics via feed processing: Implications for growth and metabolism in weaned pigs"

    Article Title: Modulating starch digestion kinetics via feed processing: Implications for growth and metabolism in weaned pigs

    Journal: Animal Nutrition

    doi: 10.1016/j.aninu.2025.08.011

    Effect of corn processing on relative mRNA level for mTOR , 4E-BP1 , p70S6K and protein abundances of mTOR, phosphorylated mTOR (p-mTOR), eukaryotic initiation factor 4E-binding protein 1 (4E-BP1), phosphorylated 4E-BP1 (p-4E-BP1), p70 ribosomal protein S6 kinase (p70S6K) and phosphorylated p70S6K (p-p70S6K) in the longissimus thoracis of piglets. Results are presented as means ± standard error of the mean (SEM), n = 4. Data columns with different letters were significantly different ( P ≤ 0.05).
    Figure Legend Snippet: Effect of corn processing on relative mRNA level for mTOR , 4E-BP1 , p70S6K and protein abundances of mTOR, phosphorylated mTOR (p-mTOR), eukaryotic initiation factor 4E-binding protein 1 (4E-BP1), phosphorylated 4E-BP1 (p-4E-BP1), p70 ribosomal protein S6 kinase (p70S6K) and phosphorylated p70S6K (p-p70S6K) in the longissimus thoracis of piglets. Results are presented as means ± standard error of the mean (SEM), n = 4. Data columns with different letters were significantly different ( P ≤ 0.05).

    Techniques Used: Binding Assay

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    Incubation:

    Article Title: Hyperactivation of autophagy contributes to high-intensity exercise-induced atrial fibrillation by activating cardiac necroptosis and inflammatory responses.
    Article Snippet: Objective: Our previous research indicated that moderate exercise protected against atrial fibrillation (AF) susceptibility in mice.. However, the precise dose-dependent effects of exercise intensity on AF and the key regulatory mechanisms involved remained unclear.. Methods: C57BL/6 J mice were subjected to an 8-week exercise regimen consisting of either moderate-intensity exercise (60 min/session, once daily) or high-intensity exercise (90 min/session, twice daily).

    Article Title: Morinda officinalis oligosaccharides improves sperm motility via the gut microbiota and the IGF-1/PI3K/mTOR signaling pathway
    Article Snippet: Equal protein (30 μg/lane) was separated on 10% SDS-PAGE and transferred to PVDF membranes (Millipore, IPVH00010). .. After blocking with 5% BSA at room temperature for 1 h, membranes were incubated overnight at 4°C with primary antibodies: Akt (Cell Signaling Technology, 9272, 1: 1,000), phosphorylated Akt (Cell Signaling Technology, 9271, 1: 1,000), IGF-1 (Cell Signaling Technology, 73034, 1: 1,000), PI3K (Cell Signaling Technology, 4257, 1: 1,000), phosphorylated PI3K (p-PI3K; Cell Signaling Technology, 17366, 1: 1,000), mTOR (Cell Signaling Technology, 2983, 1: 1,000), phosphorylated mTOR (p-mTOR; Cell Signaling Technology, 5536, 1: 1,000), Cleaved Caspase-3 (Cell Signaling Technology, 9661, 1: 1,000) and GAPDH (Abcam, ab128915, 1: 10,000). .. HRP-conjugated anti-rabbit secondary antibody (Cell Signaling Technology, 7074, 1: 1,000) was applied for 1 h at 37°C.

    Article Title: Modulating starch digestion kinetics via feed processing: Implications for growth and metabolism in weaned pigs
    Article Snippet: .. The membranes were blocked at room temperature, followed by 213 incubation at 4 °C overnight with the following primary antibodies: mammalian target 214 of rapamycin (mTOR, catalog No. 2983, Cell Signaling Technology, Danvers, MA, 215 USA), phosphorylated mTOR (p-mTOR, catalog No. 5536, Cell Signaling 216 Technology, Danvers, MA, USA), eukaryotic initiation factor 4E-binding protein 1 217 (4E-BP1, catalog No. 9644, Cell Signaling Technology, Danvers, MA, USA), 218 phosphorylated 4E-BP1 (p-4E-BP1, catalog No. 2855, Cell Signaling Technology, 219 Danvers, MA, USA), p70 ribosomal protein S6 kinase (p70S6K, catalog No. 2708, 220 Cell Signaling Technology, Danvers, MA, USA), phosphorylated p70S6K (p-p70S6K, 221 catalog No. 9234, Cell Signaling Technology, Danvers, MA, USA), and 222 glyceraldehyde-3-phosphate dehydrogenase (GAPDH, catalog No. 5174, Cell 223 Signaling Technology, Danvers, MA, USA). ..

    Article Title: Modulating starch digestion kinetics via feed processing: Implications for growth and metabolism in weaned pigs
    Article Snippet: .. The membranes were blocked at room temperature, followed by incubation at 4 °C overnight with the following primary antibodies: mammalian target of rapamycin (mTOR, catalog No. 2983, Cell Signaling Technology, Danvers, MA, USA), phosphorylated mTOR (p-mTOR, catalog No. 5536, Cell Signaling Technology, Danvers, MA, USA), eukaryotic initiation factor 4E-binding protein 1 (4E-BP1, catalog No. 9644, Cell Signaling Technology, Danvers, MA, USA), phosphorylated 4E-BP1 (p-4E-BP1, catalog No. 2855, Cell Signaling Technology, Danvers, MA, USA), p70 ribosomal protein S6 kinase (p70S6K, catalog No. 2708, Cell Signaling Technology, Danvers, MA, USA), phosphorylated p70S6K (p-p70S6K, catalog No. 9234, Cell Signaling Technology, Danvers, MA, USA), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, catalog No. 5174, Cell Signaling Technology, Danvers, MA, USA). ..

    Blocking Assay:

    Article Title: Morinda officinalis oligosaccharides improves sperm motility via the gut microbiota and the IGF-1/PI3K/mTOR signaling pathway
    Article Snippet: Equal protein (30 μg/lane) was separated on 10% SDS-PAGE and transferred to PVDF membranes (Millipore, IPVH00010). .. After blocking with 5% BSA at room temperature for 1 h, membranes were incubated overnight at 4°C with primary antibodies: Akt (Cell Signaling Technology, 9272, 1: 1,000), phosphorylated Akt (Cell Signaling Technology, 9271, 1: 1,000), IGF-1 (Cell Signaling Technology, 73034, 1: 1,000), PI3K (Cell Signaling Technology, 4257, 1: 1,000), phosphorylated PI3K (p-PI3K; Cell Signaling Technology, 17366, 1: 1,000), mTOR (Cell Signaling Technology, 2983, 1: 1,000), phosphorylated mTOR (p-mTOR; Cell Signaling Technology, 5536, 1: 1,000), Cleaved Caspase-3 (Cell Signaling Technology, 9661, 1: 1,000) and GAPDH (Abcam, ab128915, 1: 10,000). .. HRP-conjugated anti-rabbit secondary antibody (Cell Signaling Technology, 7074, 1: 1,000) was applied for 1 h at 37°C.



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    Image Search Results


    Effect of corn processing on relative mRNA level for mTOR , 4E-BP1 , p70S6K and protein abundances of mTOR, phosphorylated mTOR (p-mTOR), eukaryotic initiation factor 4E-binding protein 1 (4E-BP1), phosphorylated 4E-BP1 (p-4E-BP1), p70 ribosomal protein S6 kinase (p70S6K) and phosphorylated p70S6K (p-p70S6K) in the longissimus thoracis of piglets. Results are presented as means ± standard error of the mean (SEM), n = 4. Data columns with different letters were significantly different ( P ≤ 0.05).

    Journal: Animal Nutrition

    Article Title: Modulating starch digestion kinetics via feed processing: Implications for growth and metabolism in weaned pigs

    doi: 10.1016/j.aninu.2025.08.011

    Figure Lengend Snippet: Effect of corn processing on relative mRNA level for mTOR , 4E-BP1 , p70S6K and protein abundances of mTOR, phosphorylated mTOR (p-mTOR), eukaryotic initiation factor 4E-binding protein 1 (4E-BP1), phosphorylated 4E-BP1 (p-4E-BP1), p70 ribosomal protein S6 kinase (p70S6K) and phosphorylated p70S6K (p-p70S6K) in the longissimus thoracis of piglets. Results are presented as means ± standard error of the mean (SEM), n = 4. Data columns with different letters were significantly different ( P ≤ 0.05).

    Article Snippet: The membranes were blocked at room temperature, followed by incubation at 4 °C overnight with the following primary antibodies: mammalian target of rapamycin (mTOR, catalog No. 2983, Cell Signaling Technology, Danvers, MA, USA), phosphorylated mTOR (p-mTOR, catalog No. 5536, Cell Signaling Technology, Danvers, MA, USA), eukaryotic initiation factor 4E-binding protein 1 (4E-BP1, catalog No. 9644, Cell Signaling Technology, Danvers, MA, USA), phosphorylated 4E-BP1 (p-4E-BP1, catalog No. 2855, Cell Signaling Technology, Danvers, MA, USA), p70 ribosomal protein S6 kinase (p70S6K, catalog No. 2708, Cell Signaling Technology, Danvers, MA, USA), phosphorylated p70S6K (p-p70S6K, catalog No. 9234, Cell Signaling Technology, Danvers, MA, USA), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, catalog No. 5174, Cell Signaling Technology, Danvers, MA, USA).

    Techniques: Binding Assay